Wilcoxon or t-test? (can I pair or not)

mulecc

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Apr 10, 2016
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Say I have an experiment using insects. I'm looking at the wing and leg muscles of said insects, and how the ATPase activity in them is affected by various calcium concentrations.
Data was collected by multiple groups in multiple years from 1980-1999.
20 locusts were chosen, and one mixture prepared with wing muscle from the 20 locusts, another prepared with leg muscle from the same 20 locusts, and then each of these had ATPase activity measured subjected to calcium concentration changes.
My question is, in analysing these data, are they paired?
As you've got one mixture of all the leg muscles say, done in 1989, and you have a mixture of all the flight muscles from the same 20 insects done in 1989, as it's a mixture of either wing or leg muscles from the same locusts, can that in itself mean it's sort of averaged for all of them, and because they were done in the same year from the same insects they can be paired?
Or can you only use the paired test if it's simply dealing with individual insects on a one by one basis?
 
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